期刊
MUSCLE & NERVE
卷 44, 期 6, 页码 968-977出版社
WILEY
DOI: 10.1002/mus.22224
关键词
C2C12; cell density; community effect; contact inhibition; myogenesis
资金
- Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government [23592156]
- Grants-in-Aid for Scientific Research [23592156, 22500328] Funding Source: KAKEN
Introduction: Community effect is a phenomenon caused by cell-cell communication during myogenesis. In myogenic C2C12 cells in vitro, the confluent phase is needed for myogenesis induction. Methods: To examine the cell-density effect, growth kinetics and myogenic differentiation were investigated in cells plated at four different cell densities. Results: We found that expression of a myogenic differentiation marker was high in a density-dependent manner. At high density, where cell-cell contact was obvious, contact inhibition after the proliferation stage was accompanied by microarray findings demonstrating upregulation of negative regulating cell-cycle markers, including CDKI p21 and the muscle differentiation markers MyoD and myogenin. Interestingly, developmentally regulated protein expression (drebrin) protein expression was also upregulated in a density-dependent manner. Conclusions: These results suggest that contact inhibition after the proliferation stage may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion. Muscle Nerve 44: 968-977, 2011
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