期刊
MUSCLE & NERVE
卷 43, 期 2, 页码 283-286出版社
WILEY
DOI: 10.1002/mus.21873
关键词
Duchenne muscular dystrophy; Genotyping; mdx; mdx3cv; mdx4cv; PCR
资金
- National Institutes of Health [AR-49419]
- Muscular Dystrophy Association
mdx, mdx3cv, and mdx4cv mice are among the most commonly used models for the study of Duchenne muscular dystrophy. Their disease is caused by point mutations in the dystrophin gene. Despite widespread use of these models, genotyping has not always been straightforward. Current methods require multiple polymerase chain reactions (PCRs), post-PCR manipulations, and/or special equipment/reagents. Herein we report a simple, robust PCR genotyping method based on primer competition. This approach could also be applied in genotyping other point-mutation models. Muscle Nerve 43: 283-286, 2011
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