期刊
MULTIPLE SCLEROSIS JOURNAL
卷 15, 期 4, 页码 455-464出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1352458508100047
关键词
2D-gel electrophoresis; blood; mass spectrometry; multiple sclerosis; proteomics
资金
- Montel Williams MS Foundation
- Office of the Vice President for Research
- Office of Scientific Affairs at Stony Brook University
- NASA [NAG9-1527]
- NMSS
- National Center for Research Resources [M01-RR10710]
The diagnosis of pediatric multiple sclerosis (MS) is challenging due to its low frequency and the overlap with other acquired childhood demyelinating disorders of the central nervous system. To identify potential protein biomarkers which could facilitate the diagnosis, we used two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry to identify proteins associated with pediatric MS. Plasma samples from nine children with MS and nine healthy subjects, matched in aggregate by age and gender, were analyzed for differences in their patterns of protein expression. We found 12 proteins that were significantly up regulated in the pediatric MS group: alpha-1-acid-glycoprotein 1, alpha-1-B-glycoprotein, transthyretin, apoliprotein-C-III, serum amyloid P component, complement factor-I, clusterin, gelsolin, hemopexin, kininogen-1, hCG1993037-isoform, and vitamin D-binding protein. These results show that 2-DE in combination with mass spectrometry is a highly sensitive technique for the identification of blood-based biomarkers. This proteomic approach could lead to a new panel of diagnostic and prognostic markers in pediatric MS. Multiple Sclerosis 2009; 15: 455-464. http://msj.sagepub.com
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据