期刊
MULTIPLE SCLEROSIS
卷 14, 期 6, 页码 739-748出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1352458508088918
关键词
ELISA; glatiramer acetate; inducible nitric oxide synthase; nitric oxide; peripheral blood adherent mononuclear cells; peroxynitrite; relapsing-remitting multiple sclerosis; superoxide anion; western blot
Free radicals have been found in high concentrations within inflammatory multiple sclerosis (MS) lesions. The superoxide anion (O-2(-)) reacts rapidly with nitric oxide (NO), producing peroxynitrite (ONOO-). Glatiramer acetate (GA) is a specific MS immunomodulator that induces the synthesis of Th2 cytokines, and reduces the frequency of relapses and the formation of active brain lesions. Proinflammatory cytokines could play a role in free radicals production in the peripheral immune system as well as in the central nervous system (CNS). The effect of GA on iNOS, superoxide radicals (O-2(-)) and 3-nitrotyrosine production by peripheral blood adherent mononuclear cells (PBAMs) was assessed. Our findings demonstrate that in vitro GA reduced spontaneous and LPS-induced iNOS, 3-nitrotyrosine, NO and O-2-production, and that similar inhibition can be demonstrated ex vivo in mononuclear cells obtained from GA-treated patients. The inhibition of the production of free radicals in PBAMs may represent a new therapeutic mechanism against inflammation during MS.
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