期刊
MUCOSAL IMMUNOLOGY
卷 6, 期 2, 页码 427-434出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/mi.2012.87
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资金
- NIH [T32AI060523, R21AI094584, P01AI082971]
- Collaboration for AIDS Vaccine Discovery
- Bill and Melinda Gates Foundation [OPP1031734, 38643]
- Bill and Melinda Gates Foundation [OPP1031734] Funding Source: Bill and Melinda Gates Foundation
Cervical and vaginal epithelia are primary barriers against HIV type I (HIV-1) entry during male-to-female transmission. Cervical mucus (CM) is produced by the endocervix and forms a layer locally as well as in the vaginal compartment in the form of cervicovaginal mucus (CVM). To study the potential barrier function of each mucus type during HIV-1 transmission, we quantified HIV-1 mobility in CM and CVM ex vivo using fluorescent microscopy. Virions and 200-nm PEGylated beads were digitally tracked and mean-squared displacement was calculated. The mobility of beads increased significantly in CVM compared with CM, consistent with the known decreased mucin concentration of CVM. Unexpectedly, HIV-1 diffusion was significantly hindered in the same CVM samples in which bead diffusion was unhindered. Inhibition of virus transport was envelope-independent. Our results reveal a previously unknown activity in CVM that is capable of impeding HIV-1 mobility to enhance mucosal barrier function.
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