期刊
AQUATIC TOXICOLOGY
卷 165, 期 -, 页码 64-72出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2015.05.012
关键词
Microalga; Atrazine; Cytotoxicity; RNA-Seq; Flow cytometry
资金
- Spanish Ministerio de Economia y Competitividad [CGL2010-15993/BOS]
- Xunta de Galicia (Spain)
Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3 and 24 h. Physiological parameters related to cellular energy status, such as cellular activity and mitochondrial and cytoplasmic membrane potentials, monitored by flow cytometry, were altered in microalgal cells exposed to 0.25 mu M of atrazine. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 12 differentially expressed genes between control cultures and atrazine-exposed cultures at both tested times. Many cellular processes were affected, but the most significant changes were observed in genes implicated in amino acid catabolism and respiratory cellular process. Obtained results suggest that photosynthesis inhibition by atrazine leads cells to get energy through a heterotrophic metabolism to maintain their viability. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据