Bioactive Constituents from the Aerial Parts of Pluchea indica Less

Four new thiophenes, (3 '' R)-pluthiophenol (1), (3 '' R)-pluthiophenol-4 ''-acetate (2), 3 ''-ethoxy-(3 '' S)-pluthiophenol (3), 3 ''-ethoxy-(3 '' S)-pluthiophenol-4 ''-acetate (4), together with twenty-five known compounds were obtained from the 70% ethanol-water extract of the aerial parts of Pluchea indica Less. Their structures were elucidated by spectroscopic methods. Among the known isolates, compounds 7, 8, 11, 14, 15, 18, 20, 23, 25-27 were isolated from Asteraceae family firstly, while compounds 6, 9, 10, 12, 13, 16, 19, 21, 28 were isolated from Pluchea genus for the first time. Meanwhile, compounds 1, 2, 10, 13, 18, 23 displayed significant inhibitory activities on LPS-induced NO production at 40 mu M from RAW 264.7 macrophages, while compounds 3, 4, 26-29 possessed moderate inhibitory effects.