期刊
MOLECULES
卷 19, 期 6, 页码 7341-7355出版社
MDPI AG
DOI: 10.3390/molecules19067341
关键词
glutamate oxidase; chitosan; glutaraldehyde; glutamate; enzyme immobilization; microelectrode; micromachining; rat; hypothalamus
资金
- National Research Council of the Republic of China (Taiwan) [NSC 102-2221-E-011-117, NSC 102-2314-B-038-025-MY3]
Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (<2 s) and lower detection limit (2.5 +/- 1.1 mu M) compared to that prepared by Method 2 (response time: <5 sec and detection limit: 6.5 +/- 1.7 mu M); glutamate sensors prepared by Method 2 have a larger linear detection range (20-352 mu M) and higher sensitivity (86.8 +/- 8.8 nA.M-1.cm(-2), N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 mu M and sensitivity: 34.9 +/- 4.8 nA.M-1 cm(-2), N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.
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