期刊
MOLECULES
卷 19, 期 3, 页码 3149-3159出版社
MDPI AG
DOI: 10.3390/molecules19033149
关键词
cancer stem cell; breast cancer; anoikis
资金
- Department of Health, Executive Yuan, R.O.C. (TAIWAN) [DOH102-TD-C-111-002]
- NSYSU-KMU Joint Research Project [NSYSUKMU 2013-P019]
- Ministry of Health and Welfare of the Republic of China [MOHW103-TD-B-111-05]
- [NSYSU-KMU 103-p014]
- [NSC102-2622-B-037-003-CC2]
Cancer stem cells are a subset of cancer cells that initiate the growth of tumors. Low levels of cancer stem cells also exist in established cancer cell lines, and can be enriched in serum-free tumorsphere cultures. Since cancer stem cells have been reported to be resilient to common chemotherapeutic drugs in comparison to regular cancer cells, screening for compounds selectively targeting cancer stem cells may provide an effective therapeutic strategy. We found that 5-azacytidine (5-AzaC) selectively induced anoikis of MCF-7 in suspension cultures with an EC50 of 8.014 mu M, and effectively inhibited tumorsphere formation, as well as the migration and matrix metalloproteinases-9 (MMP-9) activity of MCF-7 cells. Furthermore, 5-AzaC and radiation collaboratively inhibited MCF-7 tumorsphere formation at clinically relevant radiation doses. Investigating the underlying mechanism may provide insight into signaling pathways crucial for cancer stem cell survival and pave the way to novel potential therapeutic targets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据