期刊
MOLECULES
卷 19, 期 9, 页码 14221-14234出版社
MDPI
DOI: 10.3390/molecules190914221
关键词
dibenzo-alpha-pyrone; botrallin; TMC-264; endophytic fungus; Hyalodendriella sp Ponipodef12; fermentation; macroporous resin; in situ resin adsorption
资金
- National Basic Research Programs of China [2013GB127800, 2010CB126105]
- Hi-Tech R&D Program of China [2011AA10A202]
Hyalodendriella sp. Ponipodef12, an endophytic fungus from the hybrid Neva of Populus deltoides x P. nigra, is a high producer of the bioactive dibenzo-alpha-pyrones botrallin and TMC-264. However, both the botrallin and TMC-264 produced by Hyalodendriella sp. Ponipodef12 were retained as both intracellular and extracellular products. The aim of this study was to evaluate an in situ macroporous resin adsorption for enhancement of botrallin and TMC-264 production in mycelial liquid culture of Hyalodendriella sp. Ponipodef12. Production of botrallin and TMC-264 was most effectively enhanced by macroporous resin DM-301 among the thirteen nonionic macroporous resins tested. The highest botrallin yield (51.47 mg/L, which was 2.29-fold higher than the control at 22.49 mg/L) was obtained by adding resin DM-301 at 4.38% (g/mL) to the culture broth on day 24 and allowing a period of 4 days for adsorption. The highest TMC-264 yield reached 47.74 mg/L, which was 11.76-fold higher than that of the control (4.06 mg/L), and was achieved by adding DM-301 resin at 4.38% (w/v) in the culture broth on day 24 and allowing a period of 6 days for adsorption. The results show that in situ resin adsorption is an effective strategy for enhancing production of botrallin and TMC-264, and also for facilitating their recovery from mycelial liquid culture of Hyalodendriella sp. Ponipodef12.
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