期刊
MOLECULES
卷 18, 期 2, 页码 2222-2235出版社
MDPI
DOI: 10.3390/molecules18022222
关键词
aflatoxins; immunoaffinity column; amino-silica gel; microparticle conjugate; agri-products; HPLC
资金
- National Natural Science Foundation of China [31201447]
- Opening Project of Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture [2012011]
- China Agriculture Research System [CARS-13]
This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G(1), B-1, G(2) and B-2 were 0.03, 0.07, 0.05 and 0.09 mu g.kg(-1), and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 mu g.kg(-1), respectively. The recoveries of aflatoxins in samples were 90.1%-104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 mu g.kg(-1).
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