期刊
MOLECULES
卷 18, 期 10, 页码 12916-12936出版社
MDPI
DOI: 10.3390/molecules181012916
关键词
P. polyphylla Smith var. chinensis (Franch.) Hara; steroidal saponins; immunostimulation; inflammation; apoptosis
资金
- Foundation of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Jiangsu Administration of Traditional Chinese Medicine [LJ200908]
- Natioanl Clinic Research Base of Triditional Chinese Medicine [JD11030]
- National Natural Science Foundation of China [81202954]
- Jiangsu Chinese Medicine Leading Talent
P. polyphylla Smith var. chinensis (Franch.) Hara (PPSCFH) has been used as medicinal Paris for the prevention and treatment of cancers in China for thousands of years. Its main components, steroidal saponins (PRS), have been confirmed to inhibit tumor growth. In the present study, the immunostimulation of PRS was investigated in Lewis bearing-C57BL/6 mice while the induction of apoptosis in A549 cells was also studied. The treatment with PRS (2.5, 5.0 and 7.5 mg/kg) significantly inhibited tumor, volume, and weight in the C57BL/6 mice. The rates of inhibition of PRS (at 2.5, 5.0 and 7.5 mg/kg) were 26.49 +/- 17.30%, 40.32 +/- 18.91% and 54.94 +/- 16.48%, respectively. The spleen and thymus indexes were increased remarkably, while the levels of inflammatory cytokines including TNF-alpha, IL-8 and IL-10 in serum were decreased according to ELISA assays. For A549 cells, Hoechst 33342 staining and annexin V/PI by flow cytometry showed that PRS (0.25, 0.50 and 0.75 mg/mL) induced nuclear changes of A549 cells with DNA condensation and fragmentations of chromatin, as well as inducing apoptosis. Furthermore, PRS could also attenuate the over-generation of intracellular ROS. Western blotting analysis showed a significant decrease on the expressions of proinflammatory cytokines MCP-1, IL-6 and TGF-beta 1, as well as cell adhesion molecule ICAM-1, by treatment with PRS. Our results demonstrated that the inhibition of PRS on tumor growth might be associated with the amelioration of inflammation responses, induction of apoptosis, as well as the decrease of ROS. These results suggested that PRS implied a potential therapeutic effect in the lung cancer treatment.
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