期刊
MOLECULES
卷 18, 期 8, 页码 8786-8798出版社
MDPI
DOI: 10.3390/molecules18088786
关键词
isoliquiritigenin; antitumour; cyclophosphamide; genotoxic effect
资金
- National Natural Science Foundation of China [81260338]
- Xinjiang Production and Construction Corps Fund for Distinguished Young Scientists [2011CD006]
- International Cooperation Project [2012BC001]
- Major State Basic Research Development Program [2010CB535003]
The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5-25 mu g/mL) or CP (0.25-1.25 mg/mL) alone or with combination of ISL (5-25 mu g/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.
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