期刊
MOLECULES
卷 17, 期 1, 页码 753-761出版社
MDPI
DOI: 10.3390/molecules17010753
关键词
okara; polysaccharide; structure; isolation
资金
- Key Scientific and Technological Project of Henan Province [102102110031]
- Ministry of Human Resources and Social Security
Okara is a byproduct generated during tofu or soymilk production processes. Crude polysaccharide (yield 56.8%) was isolated by removing fat, protein and low molecular weight carbohydrates from initial okara. Crude okara polysaccharide was further divided into four soluble fractions and an insoluble residue fraction by extracting with 0.05 M EDTA + NH4 oxalate, 0.05 M NaOH, 1 M NaOH and 4 M NaOH, with yields of 7.7%, 3.6%, 20.7%, 16.0% and 27.9%, respectively. Arabinose, galactose, galacturonic acid, xylose and glucose (only for the insoluble fraction) were the major constituent sugars. The primary sugar residues of okara polysaccharides were 1,4-linked beta-galactopyranose, 1,5- and 1,3-linked alpha-arabinofuranose, 1,5-linked alpha-xylofuranose, 1,2-linked, 1,2,4-linked and terminal alpha-rhamnopyranose (or fucopyranose), and 1,4-linked beta-glucopyranose (only for the insoluble fraction), indicating okara polysaccharides might contain galactan, arabinan, arabinogalactan, xylogalacturonan, rhamnogalacturonan, xylan, xyloglucan and cellulose.
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