期刊
MOLECULES
卷 17, 期 9, 页码 10916-10927出版社
MDPI
DOI: 10.3390/molecules170910916
关键词
oral cancer; apoptosis; ROS; glutathione; mitochondrial membrane potential; marine natural product
资金
- National Science Council of Taiwan [NSC98-2314-B-037-035]
- Department of Health [DOH101-TD-C-111-002]
- Kaohsiung Medical University Research Foundation [KMUER014, KMU-M110001]
- NSYSU-KMU JOINT RESEARCH PROJECT [NSYSUKMU 101-006]
The water extract of Gracilaria tenuistipitata have been found to be protective against oxidative stress-induced cellular DNA damage, but the biological function of the ethanolic extracts of G. tenuistipitata (EEGT) is still unknown. In this study, the effect of EEGT on oral squamous cell cancer (OSCC) Ca9-22 cell line was examined in terms of the cell proliferation and oxidative stress responses. The cell viability of EEGT-treated OSCC cells was significantly reduced in a dose-response manner (p < 0.0001). The annexin V intensity and pan-caspase activity of EEGT-treated OSCC cells were significantly increased in a dose-response manner (p < 0.05 to 0.0001). EEGT significantly increased the reactive oxygen species (ROS) level (p < 0.0001) and decreased the glutathione (GSH) level (p < 0.01) in a dose-response manner. The mitochondrial membrane potential (MMP) of EEGT-treated OSCC cells was significantly decreased in a dose-response manner (p < 0.005). In conclusion, we have demonstrated that EEGT induced the growth inhibition and apoptosis of OSCC cells, which was accompanied by ROS increase, GSH depletion, caspase activation, and mitochondrial depolarization. Therefore, EEGT may have potent antitumor effect against oral cancer cells.
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