Spiculisporic Acids B-D, Three New γ-Butenolide Derivatives from a Sea Urchin-Derived Fungus Aspergillus sp HDf2

Three new gamma-butenolide derivatives 1-3, named spiculisporic acids B-D, were isolated from the culture of Aspergillus sp. HDf2, a marine-derived fungus that resides in the sea urchin, Anthocidaris crassispina. The structures of 1-3 were elucidated on the basis of spectroscopic methods, including MS and 2D NMR techniques. Their in vitro cytotoxic activities against two cell lines (SGC-7901, human gastric adenocarcinoma and SPC-A-1, human lung adenocarcinoma) and inhibitory activities against Staphylococcus aureus ATCC 51650 were investigated.