期刊
MOLECULES
卷 17, 期 3, 页码 2437-2445出版社
MDPI
DOI: 10.3390/molecules17032437
关键词
antiviral; dengue; flavones; flavonoid; molecule
资金
- Ministry of Science, Technology, and Innovation of Malaysia (Malaysia Genome Institute initiative) [07-05-MGI-GMB015]
- Ministry of Higher Education
- University of Malaya [BK012-2011A]
This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 mu g/mL) significantly increased the number of DENV-2 foci by 35.66% +/- 1.52 and 49.66% +/- 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33% +/- 2.11 and 89% +/- 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 mu g/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 mu g/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.
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