期刊
MOLECULES
卷 17, 期 12, 页码 13813-13824出版社
MDPI AG
DOI: 10.3390/molecules171213813
关键词
lipase; transesterification; prodrug; immobilization of enzymes
资金
- Institute of Organic Chemistry and Biochemistry [AS CR RVO: 61388963]
- Academy of Science of the Czech Republic [M200551203]
Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl) adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immobilized enzymes were compared with commercially available lipases (lipase from hog pancreas, Aspergillus niger, Candida antarctica, Pseudomonas fluorescens). Lipase from Candida antarctica was found to be the most efficient enzyme regarding chemical yield of the desired products, while transesterification by lipase from Aspergillus niger resulted in lower yields.
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