期刊
MOLECULES
卷 16, 期 4, 页码 3010-3017出版社
MDPI
DOI: 10.3390/molecules16043010
关键词
hydrophobic cavity; cyclodextrin; pullulanase; complex; fluorescence spectra
资金
- International Cooperation Project of China and Netherlands [2007DFA31120]
- National Natural Science Foundation of China [20976070]
- Nature Science Foundation of Jiangsu Province [BK2008003, BK2009069]
The effects of cyclodextrins and derivatives on the activity and structure of pullulanase were investigated in this study. Our results showed that cyclodextrins and derivatives decreased the activity of pullulanase. This decrease was attributed to the interaction between the hydrophobic cavities of cyclodextrins and pullulanase. The hydrophobic cavity was confirmed to encapsulate the groups of pullulanase molecules by the addition of competitive guests. The results obtained from fluorescence spectroscopy analysis showed that beta-CD showed more efficient interactions with pullulanase molecules and the side chain groups of cyclodextrin significantly prevented the interaction between the hydrophobic cavities of beta-CD and pullulanase molecules. These findings suggest that the geometric dimension of hydrophobic cavities was crucial for matching between cyclodextrins and pullulanase and steric hindrance caused by side chains led to the decrease of the interaction.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据