期刊
MOLECULES
卷 16, 期 4, 页码 3212-3221出版社
MDPI AG
DOI: 10.3390/molecules16043212
关键词
kazinol Q; copper; DNA breakage; ROS
资金
- National Science council of Republic of China [NSC 97-2320-B-143-001]
The ability of the flavan kazinol Q (KQ) to induce DNA breakage in the presence of Cu(II) was examined by agarose gel electrophoresis using supercoiled plasmid DNA. In KQ-mediated DNA breakage reaction, the involvement of reactive oxygen species (ROS), H(2)O(2) and O(2)(-) was established by the inhibition of DNA breakage by catalase and revealed DNA breakage by superoxide dismutase (SOD). The cell viability of gastric carcinoma SCM-1 cells treated with various concentrations of KQ was significantly decreased by cotreatment with Cu(II). Treatment of SCM-1 cells with 300 mu M Cu(II) enhanced the necrosis induced by 100 mu M KQ. Treatment of SCM-1 cells with 100 mu M KQ in the presence of 300 mu M Cu(II) increased the generation of H(2)O(2). Taken together, the above finding suggested that KQ cotreatment with Cu(II) produced increased amounts of H(2)O(2), thus enhancing subsequent cell death due to necrosis.
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