期刊
MOLECULES
卷 15, 期 11, 页码 7961-7970出版社
MDPI
DOI: 10.3390/molecules15117961
关键词
endophytic fungus; Pichia guilliermondii; Paris polyphylla var. yunnanensis; steroids; helvolic acid; antimicrobial activity
资金
- Hi-Tech R&D Program of China [2006AA10Z423, 2006AA10A209]
- National Natural Science Foundation of China [30871662, 31071710]
- Natural Science Foundation of Beijing [6092015]
Three steroids and one nordammarane triterpenoid were isolated for the first time from the endophytic fungus Pichia guilliermondii Ppf9 derived from the medicinal plant Paris polyphylla var. yunnanensis. By means of physicochemical and spectrometric analysis, they were identified as ergosta-5,7,22-trienol (1), 5 alpha,8 alpha-epidioxyergosta-6,22-dien-3 beta-ol (2), ergosta-7,22-dien-3 beta,5 alpha,6 beta-triol (3), and helvolic acid (4). Both micro-dilution-colorimetric and spore germination assays were employed to evaluate their antimicrobial activity. Among them, helvolic acid (4) exhibited the strongest antibacterial activity against all test bacteria, with MIC values ranging from 1.56 mu g/mL to 50 mu g/mL, and IC50 values from 0.98 mu g/mL to 33.19 mu g/mL. It also showed strong inhibitory activity on the spore germination of Magnaporthe oryzae with an IC50 value of 7.20 mu g/mL. Among the three steroids, 5 alpha, 8 alpha-epidioxyergosta-6,22-dien-3 beta-ol (2) exhibited relatively strong antimicrobial activity. The results suggest that the endophytic fungus Pichia guillermondii Ppf9 could be a candidate for producing helvolic acid, and the metabolites from this fungus could be potentially developed as antimicrobial agents in the future.
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