期刊
MOLECULES
卷 15, 期 4, 页码 2845-2856出版社
MDPI
DOI: 10.3390/molecules15042845
关键词
plasma treatment; atelocollagen; surface modification; HaCaT; cell proliferation
资金
- Ministry of Industry and Trade of the Czech Republic [MPO 2A-1TP1/126)]
- Ministry of Education, Youth, and Sports of the Czech Republic [VZ MSM 7088352101]
- Slovenia Ministry of Higher Education, Science and Technology [P2-0082-2]
Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
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