期刊
MOLECULES
卷 13, 期 10, 页码 2408-2415出版社
MDPI
DOI: 10.3390/molecules13102408
关键词
Astragalus polysaccharides; Isolation and Purification; Molecular Weight; Monosaccharide composition
资金
- Natural Science Fund of Anhui Provincial Education Department [KJ2007B149]
- Natural Science Fund of Anhui Provincial Science and Technology Department [070413076]
Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLCGPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1: 3.45 and 1: 6.25: 17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
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