期刊
MOLECULAR THERAPY
卷 20, 期 3, 页码 534-543出版社
CELL PRESS
DOI: 10.1038/mt.2011.249
关键词
-
资金
- European Community [no HEALTH-F5-2008-222925]
- BMBF [PNP-01GS08137-5]
- German Research Council
Current gene therapy approaches for Parkinson's -disease (PD) deliver neurotrophic factors like glial cell line-derived neurotrophic factor (GDNF) or neurturin via neuronal transgene expression. Since these potent signaling-inducing neurotrophic factors can be distributed through long-distance neuronal projections to unaffected brain sites, this mode of delivery may eventually cause side effects. To explore a localized and thus potentially safer alternative for gene therapy of PD, we expressed GDNF exclusively in astrocytes and evaluated the efficacy of this approach in the mouse 1-methyl-4-phenyl-1,2,3, -6-tetrahydropyridine (MPTP) and rat -6-hydroxy-dopamine (6-OHDA) models of PD. In terms of protection of dopaminergic cell bodies and projections, dopamine (DA) synthesis and behaviour, -astrocyte-derived GDNF demonstrated the same efficacy as neuron-derived GDNF. In terms of safety, unilateral striatal GDNF expression in astrocytes did not result in delivery of bio-active GDNF to the contralateral hemispheres (potential off-target sites) as happened when GDNF was expressed in neurons. Thus, astrocytic GDNF expression represents a localized but efficient alternative to current gene therapeutic strategies for the treatment of PD, especially if viral vectors with enhanced tissue -penetration are considered. Astrocytic neurotrophic -factor expression may open new venues for neurotrophic factor-based gene therapy targeting severe diseases of the brain. Received 19 May 2011; accepted 18 October 2011; published online 15 November 2011. doi:10.1038/mt.2011.249
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据