期刊
MOLECULAR SYSTEMS BIOLOGY
卷 7, 期 -, 页码 -出版社
WILEY
DOI: 10.1038/msb.2011.66
关键词
AGB1; Arabidopsis; GPA1; heterotrimeric G-proteins; RGS1
资金
- NSF [MCB-0723515, DBI-0421683]
- Deutsche Forschungsgemeinschaft [DFG PA861/6-1, DFG UH119/6-1]
- US National Science Foundation [DBI-0923992]
- Oak Ridge National Laboratory
- US Department of Energy [DE-AC05-00OR22725]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0923992] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0723515] Funding Source: National Science Foundation
The heterotrimeric G-protein complex is minimally composed of G alpha, G beta, and G gamma subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification. Molecular Systems Biology 7: 532; published online 27 September 2011; doi:10.1038/msb.2011.66
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