期刊
MOLECULAR SYSTEMS BIOLOGY
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/msb.2011.58
关键词
functional genomics; next-generation sequencing; systems biology; transposon mutagenesis
资金
- DOE Office of Science [DE-FG02-05ER64136]
- NIH [K25 GM070972-01A2, R01, GM51426k, R01 GM32506, GM073011-04]
- Swiss National Foundation [PA00P3-126243]
- L&Th. La Roche Foundation
- Swiss National Science Foundation (SNF) [PA00P3_126243] Funding Source: Swiss National Science Foundation (SNF)
Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hypersaturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species. Molecular Systems Biology 7: 528; published online 30 August 2011; doi: 10.1038/msb.2011.58
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