期刊
MOLECULAR SYSTEMS BIOLOGY
卷 9, 期 -, 页码 -出版社
WILEY
DOI: 10.1038/msb.2013.19
关键词
computational model; glucose metabolism; insulin; network motif; temporal coding
资金
- The Creation of Fundamental Technologies for Understanding and Control of Biosystem Dynamics, CREST, from the Japan Science and Technology (JST)
- KAKENHI Scientific Research grant [21240025]
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) [11J06435]
- Human Frontier Science Project (HFSP) [RGP0061/2011]
- Daiichi-Sankyo Foundation of Life Science
- Grants-in-Aid for Scientific Research [11J06435, 22134007, 21240025] Funding Source: KAKEN
Insulin governs systemic glucose metabolism, including glycolysis, gluconeogenesis and glycogenesis, through temporal change and absolute concentration. However, how insulin-signalling pathway selectively regulates glycolysis, gluconeogenesis and glycogenesis remains to be elucidated. To address this issue, we experimentally measured metabolites in glucose metabolism in response to insulin. Step stimulation of insulin induced transient response of glycolysis and glycogenesis, and sustained response of gluconeogenesis and extracellular glucose concentration (GLC(ex)). Based on the experimental results, we constructed a simple computational model that characterises response of insulin-signalling-dependent glucose metabolism. The model revealed that the network motifs of glycolysis and glycogenesis pathways constitute a feedforward (FF) with substrate depletion and incoherent feedforward loop (iFFL), respectively, enabling glycolysis and glycogenesis responsive to temporal changes of insulin rather than its absolute concentration. In contrast, the network motifs of gluconeogenesis pathway constituted a FF inhibition, enabling gluconeogenesis responsive to absolute concentration of insulin regardless of its temporal patterns. GLC(ex) was regulated by gluconeogenesis and glycolysis. These results demonstrate the selective control mechanism of glucose metabolism by temporal patterns of insulin.
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