期刊
MOLECULAR SYSTEMS BIOLOGY
卷 10, 期 10, 页码 -出版社
WILEY
DOI: 10.15252/msb.20145470
关键词
axillary meristem; gene regulatory network; organ boundary
资金
- Ministry of Agriculture of China [2011ZX08010-002]
- National Basic Research Program of China (973 Program) [2012CB910902, 2014CB943500]
- National Natural Science Foundation of China [31171159, 31222033, 31300298, 31430010]
- Hundred Talents Program of CAS
Gene regulatory networks (GRNs) control development via cell type-specific gene expression and interactions between transcription factors (TFs) and regulatory promoter regions. Plant organ boundaries separate lateral organs from the apical meristem and harbor axillary meristems (AMs). AMs, as stem cell niches, make the shoot a ramifying system. Although AMs have important functions in plant development, our knowledge of organ boundary and AM formation remains rudimentary. Here, we generated a cellular-resolution genomewide gene expression map for low-abundance Arabidopsis thaliana organ boundary cells and constructed a genomewide protein-DNA interaction map focusing on genes affecting boundary and AM formation. The resulting GRN uncovers transcriptional signatures, predicts cellular functions, and identifies promoter hub regions that are bound by many TFs. Importantly, further experimental studies determined the regulatory effects of many TFs on their targets, identifying regulators and regulatory relationships in AM initiation. This systems biology approach thus enhances our understanding of a key developmental process.
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