期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 22, 期 4, 页码 411-420出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI-22-4-0411
关键词
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资金
- National 863 High-Tech Project of China [2006AA10A102]
- Commonweal Specialized Research Fund of China Agriculture [200803008]
- 973 Programme [2006CB101901]
- National Science foundation of China [30400260]
- Key Project of Fujian Natural Science Foundation [B0520002]
The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-t gene was delimited to an approximately 21-kb genomic fragment, in which six genes were predicted. Complementation tests of each of these six candidate genes led to the final identification of AvrPiz-t, which encodes a 108-amino-acid predicted secreted protein with unknown function and no homologues in M. oryzae or in other sequenced fungi. We found that AvrPiz-t is present in the virulent isolate GUY11 but contains a Pot3 insertion at a position 462 bp upstream from the start codon. Complementation tests of AvrPiz-t genes driven by promoters of varying length revealed that a promoter larger than 462 bp is essential to maintain the AvrPiz-t function. These results suggest that a Pot3 insertion in GUY11 might interfere with the proper function of AvrPiz-t. Additionally, we found that AvrPiz-t can suppress the programmed cell death triggered by mouse BAX protein in Nicotiana benthamiana, identifying a mechanism by which AvrPiz-t may contribute virulence of M. oryzae.
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