期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 21, 期 9, 页码 1242-1248出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI-21-9-1242
关键词
F. asiaticum
资金
- National Natural Science Foundation of China [30530510, 30571160, 30711120181]
- Ministry of Science and Technology of China [2006BAK02A12, 2007AA10Z425]
- Ministry of Agriculture of China [948]
Fusarium head blight (FHB) or scab of wheat is a devastating disease in warm and humid regions at wheat-flowering periods worldwide. Natural resistance against FHB pathogens is inadequate and the development of FHB-resistant wheat cultivars has been a challenge. Expression of pathogen-specific antibodies in plants has been proposed as a strategy for crop protection. In this study, an antibody fusion protein comprising a Fusarium-specific recombinant antibody derived from chicken and an antifungal peptide from Aspergillus giganteus was expressed in wheat as a method for protecting plants against FHB pathogens. Plants expressing the antibody fusion displayed a very significantly enhanced resistance in T2 and T3 generations upon single-floret inoculation with the macroconidia of Fusarium asiaticum, the predominant species causing FHB in China, indicating a type II resistance. Spraying inoculation further revealed an enhanced type I resistance in the transgenic wheat plants. Remarkably, more grains were produced in the transgenic plants than the nontransgenic controls. Our results demonstrated that the antibody fusion protein may be used as an effective tool for the protection of crops against FHB pathogens.
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