期刊
MOLECULAR PLANT PATHOLOGY
卷 16, 期 2, 页码 150-163出版社
WILEY
DOI: 10.1111/mpp.12168
关键词
plant phenolic compound; Rsm system; T3SS inhibitor; type III secretion system; two-component signal transduction system
资金
- National Science Foundation [EF-0332163]
- Research Growth Initiative of the University of Wisconsin-Milwaukee
- Catalyst Grant in advanced Automation of UWM Research Foundation
- National Science Foundation of China [21272029]
- 948 Project of the Ministry of Agriculture, China [2011-S8]
- 111 project, China [B13006]
The type III secretion system (T3SS) is a major virulence factor in many Gram-negative bacterial pathogens and represents a particularly appealing target for antimicrobial agents. Previous studies have shown that the plant phenolic compound p-coumaric acid (PCA) plays a role in the inhibition of T3SS expression of the phytopathogen Dickeya dadantii 3937. This study screened a series of derivatives of plant phenolic compounds and identified that trans-4-hydroxycinnamohydroxamic acid (TS103) has an eight-fold higher inhibitory potency than PCA on the T3SS of D.dadantii. The effect of TS103 on regulatory components of the T3SS was further elucidated. Our results suggest that TS103 inhibits HrpY phosphorylation and leads to reduced levels of hrpS and hrpL transcripts. In addition, through a reduction in the RNA levels of the regulatory small RNA RsmB, TS103 also inhibits hrpL at the post-transcriptional level via the rsmB-RsmA regulatory pathway. Finally, TS103 inhibits hrpL transcription and mRNA stability, which leads to reduced expression of HrpL regulon genes, such as hrpA and hrpN. To our knowledge, this is the first inhibitor to affect the T3SS through both the transcriptional and post-transcriptional pathways in the soft-rot phytopathogen D.dadantii 3937.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据