期刊
MOLECULAR PLANT PATHOLOGY
卷 13, 期 9, 页码 1149-1155出版社
WILEY
DOI: 10.1111/j.1364-3703.2012.00823.x
关键词
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资金
- Ministry of University and Research
- Regione Autonoma della Sardegna
- AGRIS SARDEGNA - Agenzia per la Ricerca in Agricoltura
High-throughput methods are needed for functional genomics analysis in Fusarium culmorum, the cause of crown and foot rot on wheat and a type B trichothecene producer. Our aim was to develop and test the efficacy of a double-component system based on the ability of the impala transposase to transactivate the miniature inverted-repeat transposable element mimp1 of Fusarium oxysporum. We report, for the first time, the application of a tagging system based on a heterologous transposon and of splinkerette-polymerase chain reaction to identify mimp1 flanking regions in the filamentous fungus F.?culmorum. Similar to previous observations in Fusarium graminearum, mimp1 transposes in F.?culmorum by a cut-and-paste mechanism into TA dinucleotides, which are duplicated on insertion. mimp1 was reinserted in open reading frames in 16.4% (i.e. 10 of 61) of the strains analysed, probably spanning throughout the entire genome of F.?culmorum. The effectiveness of the mimp1/impala double-component system for gene tagging in F.?culmorum was confirmed phenotypically for a putative aurofusarin gene. This system also allowed the identification of two genes putatively involved in oxidative stress-coping capabilities in F.?culmorum, as well as a sequence specific to this fungus, thus suggesting the valuable exploratory role of this tool.
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