期刊
MOLECULAR PLANT
卷 5, 期 6, 页码 1281-1294出版社
CELL PRESS
DOI: 10.1093/mp/sss036
关键词
ta-siRNA; miR156; PpDCL3; PpDCL4; development; moss
资金
- Landesstiftung Baden-Wurttemberg [RNS/40]
- German Federal Ministry of Education and Research [FRISYS 0313921]
- German Academic Exchange Service (DAAD)
- US National Institutes of Health [R01GMR084051]
- [GRK1305]
Trans-acting small interfering RNAs (ta-siRNAs) are plant-specific siRNAs released from TAS precursor transcripts after microRNA-dependent cleavage, conversion into double-stranded RNA, and Dicer-dependent phased processing. Like microRNAs (miRNAs), ta-siRNAs direct site-specific cleavage of target RNAs at sites of extensive complementarity. Here, we show that the DICER-LIKE 4 protein of Physcomitrella patens (PpDCL4) is essential for the biogenesis of 21 nucleotide (nt) ta-siRNAs. In Delta PpDCL4 mutants, off-sized 23 and 24-nt ta-siRNAs accumulated as the result of PpDCL3 activity. Delta PpDCL4 mutants display severe abnormalities throughout Physcomitrella development, including sterility, that were fully reversed in Delta PpDCL3/Delta PpDCL4 double-mutant plants. Therefore, PpDCL3 activity, not loss of PpDCL4 function per se, is the cause of the Delta PpDCL4 phenotypes. Additionally, we describe several new Physcomitrella trans-acting siRNA loci, three of which belong to a new family, TAS6. TAS6 loci are typified by sliced miR156 and miR529 target sites and are in close proximity to PpTAS3 loci.
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