期刊
MOLECULAR PLANT
卷 4, 期 2, 页码 310-318出版社
CELL PRESS
DOI: 10.1093/mp/ssq077
关键词
Tobacco BY-2 cells; calcium signaling; cytosolic calcium; aequorin; sphingolipids; LCBs; dihydrosphingosine; sphinganine; apoptosis; Programmed Cell Death (PCD); Reactive Oxygen Species (ROS); H2O2; oxidative burst
资金
- Centre National de la Recherche Scientifique
- Universite de Toulouse
Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid Long Chain Base (LCB) in plants, has been recently shown to induce both cytosolic and nuclear calcium transient increases and a correlated Programmed Cell Death (PCD) in tobacco BY-2 cells. In this study, in order to get deeper insight into the LCB signaling pathway leading to cell death, the putative role of Reactive Oxygen Species (ROS) has been investigated. We show that DHS triggers a rapid dose-dependent production of H2O2 that is blocked by diphenyleniodonium (DPI), indicating the involvement of NADPH oxidase(s) in the process. In addition, while DPI does not block DHS-induced calcium increases, the ROS production is inhibited by the broad spectrum calcium channel blocker lanthanum (La3+). Therefore, ROS production occurs downstream of DHS-induced Ca2+ transients. Interestingly, DHS activates expression of defense-related genes that is inhibited by both La3+ and DPI. Since DPI does not prevent DHS-induced cell death, these results strongly indicate that DHS-induced H2O2 production is not implicated in PCD mechanisms but rather would be associated to basal cell defense mechanisms.
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