期刊
MOLECULAR PHYLOGENETICS AND EVOLUTION
卷 67, 期 2, 页码 529-540出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ympev.2013.02.022
关键词
Chiharaea; COI-5P; DNA barcoding; Introgression; ITS; rbcL
资金
- Canadian Barcode of Life Network from Genome Canada through the Ontario Genomics Institute
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chair Program
- Canada Foundation for Innovation
- New Brunswick Innovation Foundation
The use of molecular markers in taxonomic studies has become a standard practice in biology. However, consensus on which markers to use at the species level is lacking because evolutionary lineages show differences in divergence rates between organellar genomes. Ideally, researchers use multiple lines of evidence when first describing a species, such as the incorporation of several molecular markers from varied genomes (mitochondrion, plastid and nucleus). This study examined species boundaries in the red algal genus Chiharaea. We used five molecular markers, with at least one marker from each genome, coupled with thorough morphological analyses. We recognized three species in Chiharaea (C.americana, C rhododactyla sp. nov., C silvae) and two forms (C americana f. americana and C americana f. bodegensis (H.W. Johansen) stat. nov.). For C americana f. americana and C americana f. bodegensis differentiation based on morphological data was reflected in the plastid-encoded large subunit of RuBisCO (rbcL), but was not concordant with either the mitochondria] cytochrome c oxidase subunit 1 (COI-5P) or nuclear internal transcribed spacer (ITS) sequence data. We suggest that this discordance is indicative of ongoing hybridization and introgression between populations of C americana f. americana and C americana f. bodegensis. In addition, we used a PCR assay with ITS specific primers to amplify multiple ITS variants for collections assignable to C americana indicating that there is genetic variability within ITS copies most likely due to introgression, crossing over and/or the retention of ancestral variants. (C) 2013 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据