期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 58, 期 5, 页码 1098-1110出版社
WILEY
DOI: 10.1002/mnfr.201300497
关键词
Fructooligosaccharides; Inulin; Monocyte; NF-B; Toll like receptor 4
资金
- Ministerio de Economia y Competividad [SAF2008-01432, AGL2008-04332, SAF2011-22922, SAF2011-22812]
- Junta de Andalucia [CTS6736, CTS164]
- Fundacion Ramon Areces
- Ministerio of Educacion
- Instituto de Salud Carlos III
ScopePrebiotic oligosaccharides are currently used in a variety of clinical settings for their effects on intestinal microbiota. Here, we have examined the direct, microbiota independent, effects of prebiotics on monocytes and T lymphocytes in vitro. Methods and resultsPrebiotics generally evoked cytokine secretion (TNF-, IL-6, and IL-10) by mouse splenocytes but inhibited LPS -induced IFN- and IL-17 release. Inulin was found to enhance LPS-induced IL-10 secretion. Splenocytes from TLR4(-/-) (where TLR is Toll-like receptor) mice showed a markedly depressed response. Conversely, in both basal and LPS-stimulated conditions, prebiotic inhibition of IFN- levels was preserved. These results suggested a predominant effect on monocytes via TLR4 ligation and possible inhibition of T cells. Hence, we studied the modulation of primary rat monocytes and T lymphocytes, focusing on fructooligosaccharides (FOS) and inulin. In monocytes, FOS and inulin induced TNF-, growth-regulated oncogene , and IL-10, but not IL-1 release. The NF-B inhibitor Bay 11-7082 fully prevented these effects. Pharmacological evidence also indicated a significant involvement of mitogen-activated protein kinase and phosphatidylinositol-3-kinase. There was little effect on T cells. FOS and inulin also generally increased TNF-, IL-1, and IL-10, but not IL-8, in human peripheral blood monocytes. ConclusionWe conclude that prebiotics may act as TLR4 ligands or as indirect TLR4 modulators to upregulate cytokine secretion in monocytes.
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