期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 58, 期 4, 页码 782-798出版社
WILEY
DOI: 10.1002/mnfr.201300369
关键词
Apoptosis; BID; Cathepsin D; Phosphatidylcholine; Sphingomyelin
资金
- Swiss National Science Foundation [SNF 31003A_127247, SNF 310030 120312]
- Zurich Center for Integrative Human Physiology (ZIHP)
- Swiss inflammatory bowel disease cohort study (SIBDC)
- Internal Grant Agency of Ministry of Health of the Czech Republic [NT 13441-4/2012]
- European Regional Development Fund-Project FNUSA-ICRC [CZ.1.05/1.1.00/02.0123]
- Abbot
- Ardeypharm
- Essex
- FALK
- Flamentera
- Novartis
- Roche
- Tillots
- UCB
- Zeller
- Swiss National Science Foundation (SNF) [31003A_127247] Funding Source: Swiss National Science Foundation (SNF)
ScopeThe major alimentary sources for the plasma membrane lipid sphingomyelin (SM) are dairy products, eggs, and meat. We recently reported that the SM metabolite ceramide induces cathepsin D mediated apoptosis in murine intestinal epithelial cells (IECs) and increases inflammation in acute colitis. We investigated the impact of SM and phosphatidylcholine on apoptosis in human IECs and point out BH3-interacting death agonist (BID) as link between cathepsin D and apoptosis. Methods and resultsHT-29 and isolated human IECs were stimulated with SM or phosphatidylcholine. SM treatment resulted in increased apoptosis. Phosphatidylcholine showed contrary effects. Western revealed higher amounts of cathepsin D and BID activation upon lipid stimulation. Western blotting revealed BID activation through SM in both an induced and a spontaneous mouse model of colitis. ConclusionDietary phospholipids may induce or abolish apoptosis in IECs and seem to play a role in the pathogenesis of inflammatory bowel diseases. This nutritional factor might be considered when evaluating the pathogenesis of inflammatory bowel diseases. Effects of SMase- and SM treatment on inflammation in dextran sulfate sodium induced animal models of colitis and in vitro experiments are discussed as controversial. Variable sources of SM, feeding techniques, and mouse strains might play a role.
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