期刊
MOLECULAR NEUROBIOLOGY
卷 50, 期 2, 页码 423-437出版社
HUMANA PRESS INC
DOI: 10.1007/s12035-014-8640-x
关键词
Neural stem cells (NSCs); Neuron; Oligodendrocytes; Astrocyte; Transplantation; Alzheimer's disease (AD); APP/PS1 transgenic mouse; NMDA; Synaptophysin; PKC zeta; TrkB; BDNF; LTP; Cognition; Amyloid
资金
- National Natural Science Foundation of China [81171297, 81200934]
Neural stem cells (NSCs) are capable of self-renewal and are multipotent. Transplantation of NSCs may represent a promising approach for treating neurodegenerative disorders associated with cognitive decline, such as Alzheimer disease (AD) characterized by extensive loss of neurons. In this study, we investigated the effect of NSC transplantation on cognitive function in the amyloid precursor protein/presenilin-1 (APP/PS1) transgenic mouse, an AD mouse model with age-dependent cognitive deficits. We found that NSCs bilaterally transplanted into hippocampal regions improved spatial learning and memory function in these mice, but did not alter A beta pathology. Immunohistochemical analyses determined that NSCs proliferated, migrated, and differentiated into three neuronal cell types. The improvement in cognitive function was correlated with enhanced long-term potentiation (LTP) and an increase in the neuron expression of proteins related to cognitive function: N-methyl-d-aspartate (NMDA) 2B unit, synaptophysin (SYP), protein kinase C zeta subtypes (PKC zeta), tyrosine receptor kinase B (TrkB), and brain-derived neurotrophic factor (BDNF). Taken together, our data indicated that injected NSCs can rescue cognitive deficits in APP/PS1 transgenic mice by replacing neuronal cell types expressing multiple cognition-related proteins that enhance LTP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据