期刊
MOLECULAR NEUROBIOLOGY
卷 51, 期 3, 页码 843-852出版社
SPRINGER
DOI: 10.1007/s12035-014-8808-4
关键词
Diabetic encephalopathy; Pathogenesis; C-13 magnetic resonance spectroscopy; Pyruvate recycling; Anaplerotic pathway
资金
- National Natural Science Foundation of China [21175099]
- Zhejiang Provincial Natural Science Foundation of China [LY14H090014]
- Zhejiang Provincial Program for the Cultivation of Health talents
- Science and Technology Foundation of Wenzhou [Y20100005]
Increasing evidence has shown that the brain is a site of diabetic end-organ damage. This study investigates cerebral metabolism and the interactions between astrocytes and neurons at different stages of diabetes to identify the potential pathogenesis of diabetic encephalopathy. [1-C-13]glucose or [2-C-13]acetate is infused into 1- and 15-week diabetic rats, the brain extracts of which are analyzed by using H-1 and C-13 magnetic resonance spectroscopy. The C-13-labeling pattern and enrichment of cerebral metabolites are also investigated. The increased C-13 incorporation in the glutamine, glutamate, and gamma-aminobutyric acid carbons from [2-C-13]acetate suggests that the astrocytic mitochondrial metabolism is enhanced in 1-week diabetic rats. By contrast, the decreased labeling from [1-C-13]glucose reflected that the neuronal mitochondrial metabolism is impaired. As diabetes developed to 15 weeks, glutamine and glutamate concentrations significantly decreased. The increased labeling of glutamine C4 but unchanged labeling of glutamate C4 from [2-C-13]acetate suggests decreased astrocyte supply to the neurons. In addition, the enhanced pyruvate recycling pathway manifested by the increased lactate C2 enrichment in 1-week diabetic rats is weakened in 15-week diabetic rats. Our study demonstrates the overall metabolism disturbances, changes in specific metabolic pathways, and interaction between astrocytes and neurons during the onset and development of diabetes. These results contribute to the mechanistic understanding of diabetes pathogenesis and evolution.
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