期刊
MOLECULAR MICROBIOLOGY
卷 94, 期 6, 页码 1242-1259出版社
WILEY
DOI: 10.1111/mmi.12833
关键词
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资金
- Science Foundation Ireland [08/IN.1/B1859, 12/1A/1570]
- EU
- Science Foundation Ireland (SFI) [08/IN.1/B1859] Funding Source: Science Foundation Ireland (SFI)
The PhoPR two-component signal transduction system controls one of the major responses to phosphate limitation in Bacillus subtilis. When activated it directs expression of phosphate scavenging enzymes, lowers synthesis of the phosphate-rich wall teichoic acid (WTA) and initiates synthesis of teichuronic acid, a non-phosphate containing replacement anionic polymer. Despite extensive knowledge of this response, the signal to which PhoR responds has not been identified. Here we report that one of the main functions of the PhoPR two-component system in B. subtilis is to monitor WTA metabolism. PhoR autokinase activity is controlled by the level of an intermediate in WTA synthesis that is sensed through the intracellular PAS domain. The pool of this intermediate generated by WTA synthesis in cells growing under phosphate-replete conditions is sufficient to inhibit PhoR autokinase activity. However WTA synthesis is lowered upon phosphate limitation by the combined effects of PhoP similar to P-mediated activation of tuaA-H transcription and repression of tagAB. These transcriptional changes combine to lower the level of the inhibitory WTA metabolite thereby increasing PhoR autokinase activity. This amplifies the PHO response with full induction being achieved similar to 90 min after the onset of phosphate limitation.
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