期刊
MOLECULAR MICROBIOLOGY
卷 94, 期 2, 页码 418-433出版社
WILEY-BLACKWELL
DOI: 10.1111/mmi.12776
关键词
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资金
- Mizutani Foundation for Glycoscience
- Swedish Research Council Formas (via CarboMat - the KTH Advanced Carbohydrate Materials Centre), faculty funding from the Michael Smith Laboratories/UBC
- Natural Sciences and Engineering Research Council of Canada (Discovery Grant)
- Canada Foundation for Innovation
- British Columbia Knowledge Development Fund
- Biotechnology and Biological Science Research Council (BBSRC) [BB/I014802/1]
- start-up funds - Provost's Office at UMBC
- BBSRC [BB/I014802/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I014802/1] Funding Source: researchfish
The degradation of plant biomass by saprophytes is an ecologically important part of the global carbon cycle, which has also inspired a vast diversity of industrial enzyme applications. The xyloglucans (XyGs) constitute a family of ubiquitous and abundant plant cell wall polysaccharides, yet the enzymology of XyG saccharification is poorly studied. Here, we present the identification and molecular characterization of a complex genetic locus that is required for xyloglucan utilization by the model saprophyte Cellvibrio japonicus. In harness, transcriptomics, reverse genetics, enzyme kinetics, and structural biology indicate that the encoded cohort of an -xylosidase, a -galactosidase, and an -l-fucosidase is specifically adapted for efficient, concerted saccharification of dicot (fucogalacto)xyloglucan oligosaccharides following import into the periplasm via an associated TonB-dependent receptor. The data support a biological model of xyloglucan degradation by C. japonicus with striking similarities - and notable differences - to the complex polysaccharide utilization loci of the Bacteroidetes.
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