期刊
MOLECULAR MICROBIOLOGY
卷 81, 期 4, 页码 952-967出版社
WILEY
DOI: 10.1111/j.1365-2958.2011.07741.x
关键词
-
资金
- PHS [R37-GM40313]
- NIH (BRTP/NCRR) (NIGMS) [P41RR02301, P41GM66326]
- University of Wisconsin
- NIH [RR02781, RR08438]
- NSF [DMB-8415048, OIA-9977486, BIR-9214394]
- USDA
In the homoacetogenic bacterium Sporomusa ovata, phenol and p-cresol are converted into alpha-ribotides, which are incorporated into biologically active cobamides (Cbas) whose lower ligand bases do not form axial co-ordination bonds with the cobalt ion of the corrin ring. Here we report the identity of two S. ovata genes that encode an enzyme that transfers the phosphoribosyl group of nicotinate mononucleotide (NaMN) to phenol or p-cresol, yielding alpha-O-glycosidic ribotides. The alluded genes were named arsA and arsB (for alpha-ribotide synthesis), arsA and arsB were isolated from a genomic DNA library of S. ovata. A positive selection strategy using an Escherichia coli strain devoid of NaMN:5,6-dimethylbenzimidazole (DMB) phosphoribosyltransferase (CobT) activity was used to isolate a fragment of S. ovata DNA that contained arsA and arsB, whose nucleotide sequences overlapped by 8 bp. SoArsAB was isolated to homogeneity, shown to be functional as a heterodimer, and to have highest activity at pH 9. SoArsAB also activated DMB to its alpha-N-glycosidic ribotide. Previously characterized CobT-like enzymes activate DMB but do not activate phenolics. NMR spectroscopy was used to confirm the incorporation of phenol into the cobamide, and mass spectrometry was used to identify SoArsAB reaction products.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据