期刊
MOLECULAR MICROBIOLOGY
卷 79, 期 1, 页码 21-34出版社
WILEY
DOI: 10.1111/j.1365-2958.2010.07439.x
关键词
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资金
- NIH [GM022778]
- Howard Hughes Medical Institute
- Spanish Ministerio de Educacion y Ciencia
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P01GM022778] Funding Source: NIH RePORTER
Ornate, large, extremophilic (OLE) RNAs are large, non-coding transcripts characterized by their ornate secondary structure and presence predominantly in Gram-positive, extremophilic bacteria. A gene for an OLE-associated protein (OAP) is almost always located immediately downstream of the OLE gene. OAP has no extensive homology to other proteins and is predicted to form multiple transmembrane domains. We show that this protein forms a ribonucleoprotein complex with OLE RNA using at least 2: 1 protein : RNA stoichiometry. A series of truncated OLE RNA constructs was used to establish that most of the RNA can be deleted without eliminating protein binding. Two primary binding sites are present within the RNA, although additional binding determinants exist and extensive structural stabilization is induced by OAP. RNA fluorescence in situ hybridization (FISH) was used in Escherichia coli to demonstrate that ribonucleoprotein complex formation localizes the RNA near cell membranes of this heterologous system. Therefore, the majority of the complex structure formed by OLE RNA may perform a biochemical function that requires membrane localization.
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