期刊
MOLECULAR MICROBIOLOGY
卷 72, 期 3, 页码 741-762出版社
WILEY
DOI: 10.1111/j.1365-2958.2009.06677.x
关键词
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资金
- Institut Pasteur
- Network of Excellence 'Europathogenomics' [LSHB-CT-2005-512061]
- NIH [2 R01 AI44212]
- German Academy of Natural Scientists Leopoldina [BMBF-LPD9901/8-101]
- PTR 185 (Institut Pasteur-Biorad)
- Marie Curie fellowship
- European Commission [MEST-CT-2005-020715]
- Bavarian Research Foundation (Bayerische Forschungsstiftung)
To transit from intra- to extracellular environments, Legionella pneumophila differentiates from a replicative/non-virulent to a transmissive/ virulent form using the two-component system LetA/LetS and the global repressor protein CsrA. While investigating how both regulators act co-ordinately we characterized two ncRNAs, RsmY and RsmZ, that link the LetA/LetS and CsrA regulatory networks. We demonstrate that LetA directly regulates their expression and show that RsmY and RsmZ are functional in Escherichia coli and are able to bind CsrA in vitro. Single mutants have no (Delta rsmY) or a little (Delta rsmZ) impact on virulence, but the Delta rsmYZ strain shows a drastic defect in intracellular growth in Acanthamoeba castellanii and THP-1 monocyte-derived macrophages. Analysis of the transcriptional programmes of the Delta letA, Delta letS and Delta rsmYZ strains revealed that the switch to the transmissive phase is partially blocked. One major difference between the Delta letA, Delta letS and Delta rsmYZ strains was that the latter synthesizes flagella. Taken together, LetA activates transcription of RsmY and RsmZ, which sequester CsrA and abolish its post-transcriptional repressive activity. However, the RsmYZ-CsrA pathway appears not to be the main or only regulatory circuit governing flagella synthesis. We suggest that rather RpoS and LetA, by influencing LetE and probably cyclic-di-GMP levels, regulate motility in L. pneumophila.
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