4.5 Article

Mutational analyses of HAMP helices suggest a dynamic bundle model of input-output signalling in chemoreceptors

期刊

MOLECULAR MICROBIOLOGY
卷 73, 期 5, 页码 801-814

出版社

WILEY
DOI: 10.1111/j.1365-2958.2009.06819.x

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资金

  1. National Institute of General Medical Sciences [GM19559]
  2. National Cancer Institute [CA42014]
  3. NATIONAL CANCER INSTITUTE [P30CA042014] Funding Source: NIH RePORTER
  4. NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM019559, R37GM019559] Funding Source: NIH RePORTER

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P>To test the gearbox model of HAMP signalling in the Escherichia coli serine receptor, Tsr, we generated a series of amino acid replacements at each residue of the AS1 and AS2 helices. The residues most critical for Tsr function defined hydrophobic packing faces consistent with a four-helix bundle. Suppression patterns of helix lesions conformed to the predicted packing layers in the bundle. Although the properties and patterns of most AS1 and AS2 lesions were consistent with both proposed gearbox structures, some mutational features specifically indicate the functional importance of an x-da bundle over an alternative a-d bundle. These genetic data suggest that HAMP signalling could simply involve changes in the stability of its x-da bundle. We propose that Tsr HAMP controls output signals by modulating destabilizing phase clashes between the AS2 helices and the adjoining kinase control helices. Our model further proposes that chemoeffectors regulate HAMP bundle stability through a control cable connection between the transmembrane segments and AS1 helices. Attractant stimuli, which cause inward piston displacements in chemoreceptors, should reduce cable tension, thereby stabilizing the HAMP bundle. This study shows how transmembrane signalling and HAMP input-output control could occur without the helix rotations central to the gearbox model.

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