期刊
MOLECULAR MICROBIOLOGY
卷 73, 期 3, 页码 409-418出版社
WILEY
DOI: 10.1111/j.1365-2958.2009.06780.x
关键词
-
资金
- National Research Initiative of the United States Department of Agriculture (USDA) Cooperative State Research, Education, and Extension Service [2005-35319-19202]
- Robert A. Welch Foundation [C-0729]
- USDA Cooperative State Research, Education, and Extension Service [2001-52104-11484]
- National Institutes of Health (NIH) Chemistry of Biological Systems Training Grant at Cornell University
Thaxtomin A, a cyclic dipeptide with a nitrated tryptophan moiety, is a phytotoxic pathogenicity determinant in scab-causing Streptomyces species that inhibits cellulose synthesis by an unknown mechanism. Thaxtomin A is produced by the action of two non-ribosomal peptide synthetase modules (TxtA and TxtB) and a complement of modifying enzymes, although the order of biosynthesis has not yet been determined. Analysis of a thaxtomin dual module knockout mutant and single module knockout mutants revealed that 4-nitrotryptophan is an intermediate in thaxtomin A biosynthesis prior to backbone assembly. The 4-nitrotryptophan represents a novel substrate for non-ribosomal peptide synthetases. Through identification of N-methyl-4-nitrotryptophan in a single module knockout and the use of adenylation domain specificity prediction software, TxtB was identified as the non-ribosomal peptide synthetase module specific for 4-nitrotryptophan.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据