期刊
MOLECULAR MEDICINE REPORTS
卷 18, 期 4, 页码 3907-3913出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2018.9423
关键词
pirfenidone; intestinal fibroblasts; proliferation; apoptosis
资金
- National Clinical Key Specialty Construction Project (General Surgery) of China [2012-649]
- Guiding Key Project of Social Development by the Fujian Provincial Science and Technology Department [2015Y0058]
Intestinal fibroblasts, the main effector cells of intestinal fibrosis, are considered to be a good target for anti-fibrotic therapy. The aim of the present study was to examine the effects of pirfenidone (PFD) on human intestinal fibroblasts (HIFs) stimulated by transforming growth factor (TGF)-1 and to explore the potential mechanism. Prior to stimulation with TGF-1 (10 ng/ml), HIFs were treated with or without PFD (1 mg/ml). Cell proliferation was determined by Cell Counting Kit (CCK)-8 and colony formation assays, and cell apoptosis was assessed using flow cytometry and a TUNEL assay. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to evaluate the mRNA and protein expressions of -smooth muscle actin (-SMA), collagen I and fibronectin. The protein expression of TGF-1/mothers against decapentaplegic homolog (Smad) and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways was evaluated by western blotting. CCK-8 and colony formation assays demonstrated that PFD significantly inhibited cell proliferation in HIFs stimulated with TGF-1. Flow cytometry and TUNEL assays revealed that PFD treatment significantly enhanced apoptosis in TGF-1-stimulated HIFs. In addition, PFD markedly reduced TGF-1-induced HIF activities, such as myofibroblast differentiation (-SMA), and collagen production (collagen I and fibronectin). These effects of PFD were mediated by the inhibition of the TGF-1/Smad and PI3K/AKT signaling pathways. Therefore, the present study demonstrated that PFD reduced TGF-1-induced fibrogenic activities of HIFs, suggesting that PFD may be a potential therapeutic agent for intestinal fibrosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据