期刊
MOLECULAR MEDICINE REPORTS
卷 9, 期 5, 页码 1629-1633出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2014.2043
关键词
steroid receptor coactivator-3; hematopoiesis; irradiation
资金
- National '863' High-tech Development Plan [2007AA02Z152]
- National Natural Science Fund of China [31071025]
- Special Fund from PLA [BSW11J009]
- National '125' Technology Support Plan [2013BAK03B05-02]
The objective of the present study was to investigate the role of the steroid receptor coactivator-3 (SRC-3) in hematopoiesis of mouse bone marrow (BM) following total body irradiation (TBI). SRC-3(-/-) mice and wild-type (WT) mice were exposed to 4.5 Gy gamma rays. Immunoblotting analysis revealed that the SRC-3 protein (p160) levels in normal BM-nucleated cells in WT were higher than in SRC-3(-/-) mice. Furthermore, peripheral blood cell counts, BM cellularity and colony-forming unit (CFU) assays were performed following irradiation. The results showed that peripheral blood cells were significantly lower in number and recovered less rapidly in irradiated SRC-3(-/-) mice as compared with control animals. BM-nucleated cell and CFU counts were significantly decreased in SRC-3(-/-) mice on the 7th and 14th day. Of note, the recovery of platelet (PLT) and megakaryocytic lineage were more depressed than the granulocytic and erythroid lineage in SRC-3(-/-) mice. In conclusion, the present study demonstrated that the hematopoietic ability in SRC-3 knockout mice is severely impaired following a sublethal dose of irradiation.
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