期刊
SCIENCE SIGNALING
卷 8, 期 406, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.aac6479
关键词
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资金
- NIH [R01DK99551]
- National Cancer Institute [PO1 (CA128814), RO1 (CA179170)]
- Hervey Family Non-Endowment Fund at the San Diego Foundation
- Melanoma Research Foundation [K99 (CA172705), T32 (CA121949)]
- Assistant Secretary of Defense for Health Affairs through the Peer-Reviewed Cancer Program [W81XWH-14-1-0127]
Melanoma is one of the most lethal skin cancers worldwide, primarily because of its propensity to metastasize. Thus, the elucidation of mechanisms that govern metastatic propensity is urgently needed. We found that protein kinase C epsilon (PKC epsilon)-mediated activation of activating transcription factor 2 (ATF2) controls the migratory and invasive behaviors of melanoma cells. PKC epsilon-dependent phosphorylation of ATF2 promoted its transcriptional repression of the gene encoding fucokinase (FUK), which mediates the fucose salvage pathway and thus global cellular protein fucosylation. In primary melanocytes and cell lines representing early-stage melanoma, the abundance of PKC epsilon-phosphorylated ATF2 was low, thereby enabling the expression of FUK and cellular protein fucosylation, which promoted cellular adhesion and reduced motility. In contrast, increased expression of the gene encoding PKC epsilon and abundance of phosphorylated, transcriptionally active ATF2 were observed in advanced-stage melanomas and correlated with decreased FUK expression, decreased cellular protein fucosylation, attenuated cell adhesion, and increased cell motility. Restoring fucosylation in mice either by dietary fucose supplementation or by genetic manipulation of murine Fuk expression attenuated primary melanoma growth, increased the number of intratumoral natural killer cells, and decreased distal metastasis in murine isograft models. Tumor microarray analysis of human melanoma specimens confirmed reduced fucosylation in metastatic tumors and a better prognosis for primary melanomas that had high abundance of fucosylation. Thus, inhibiting PKC epsilon or ATF2 or increasing protein fucosylation in tumor cells may improve clinical outcome in melanoma patients.
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