期刊
MOLECULAR MEDICINE REPORTS
卷 7, 期 3, 页码 791-798出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2013.1288
关键词
dengue virus; peripheral blood mononuclear cells; microRNAs; bioinformatic analysis
资金
- National Natural Science Foundation of China [30872350]
- Natural Science Foundation of Guangdong Province [81510008901000017, S2012010009050]
- Guangdong Province Scientific Technology Project [2010B050700008, 2011B040300022]
- Guangzhou City Scientific Technology Project [2011J4100084, 2008Z1-E221]
- Fundamental Research Funds for the Central Universities [10YKPY31]
Dengue virus (DENV) causes self-limiting dengue fever (DF), severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It is generally considered that cytokine storm leads to the increased plasma leakage characteristic of DHF/DSS. In the present study, peripheral blood mononuclear cells (PBMCs) were isolated from blood samples of healthy volunteers and infected with DENV serotype 2 (DENV2). Culture supernatants of DENV2-infected and -uninfected PBMCs were analyzed using a human cytokine array. Between a 6-12 h post-infection, levels of CCL5, IL-6 and IL-8 were markedly elevated, while those of TNF-alpha decreased. Total RNA isolated from these PBMCs was analyzed by human miRNA microarray to identify differentially expressed microRNAs (miRNAs). Quantitative reverse transcription polymerase chain reaction was used to validate 11 upregulated and 4 downregulated miRNAs. Sanger mibase, miRanda and Target Scan were used to identify 261 common predicted genes. Databases were used to identify homologous sequences on mRNAs of putative target genes that may be directly bound by the miRNAs identified. We found that cytokines and epigenetic regulators may be putative target genes of these miRNAs. Using ingenuity pathway analysis, we noted that canonical pathways, including biological regulation, may be modulated by these miRNAs.
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